Proteinexpression and purification

23 May 2004, ExploHeidelberg Teaching lab
Teaching Lab
     
EMBO
Promoting Biosciences in Europe
 
molecules in medicine
an update for the class room
 
3rd international practical workshop
for science teachers
 
Contact:
 
Dr. Thomas Wendt
ExploHeidelberg Teaching Lab
Tel.: (+49)6221 421404
Fax: (+49)6221 421410
email: [email protected]

Results:

Biorad protein assay:

group eluate # volume OD 595 mg protein concentration
1 1 40 0,46 8,50 0,21
2 40 0,21 3,80 0,10
2 1 40 0,74 14,00 0,35
2 40 0,40 7,00 0,18
3 1 20 0,87 18,00 0,90
2 20 0,40 7,00 0,35
4 1 20 0,42 7,50 0,38
2 20 0,06 1,00 0,05
5 1 40 1,01 20,00 0,50
2 40 0,71 10,50 0,26
6 1 20 0,30 5,00 0,25
2 20 0,10 2,00 0,10

Protein PAGE: